A field study of gross rates of N mineralization and nitrification and their relationships to microbial biomass and enzyme activities in soils treated with dairy effluent and ammonium fertilizer

Abstract

Abstract. Gross N mineralization and nitrification rates were measured in soils treated with dairy shed effluent (DSE) (i.e. effluent from the dairy milking shed, comprising dung, urine and water) or ammonium fertilizer (NH4Cl) under field conditions, by injecting 15N‐solution into intact soil cores. The relationships between gross mineralization rate, microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) as affected by the application of DSE and NH4Cl were also determined. During the first 16 days, gross mineralization rate in the DSE treated soil (4.3–6.1 μg N g−1 soil day−1) were significantly (P 14;< 14;0.05) higher than those in the NH4Cl treated soil (2.6–3.4 μg N g−1 soil day−1). The higher mineralization rate was probably due to the presence of readily mineralizable organic substrates in the DSE, accompanied by stimulated microbial and extracellular enzyme activities. The stable organic N compounds in the DSE were slow to mineralize and contributed little to the mineral N pool during the period of the experiment. Nitrification rates during the first 16 days were higher in the NH4Cl treated soil (1.7–1.2 μg N g−1 soil day−1) compared to the DSE treated soil (0.97–1.5 μg N g−1 soil day−1). Soil microbial biomass C and N and extracellular enzyme activities (protease, deaminase and urease) increased after the application of the DSE due to the organic substrates and nutrients applied, but declined with time, probably because of the exhaustion of the readily available substrates. The NH4Cl application did not result in any significant increases in microbial biomass C, protease or urease activities due to the lack of carbonaceous materials in the ammonium fertilizer. However, it did increase microbial biomass N and deaminase activity. Significant positive correlations were found between gross N mineralization rate and soil microbial biomass, protease, deaminase and urease activities. Nitrification rate was significantly correlated to biomass N but not to the microbial biomass C or the enzyme activities. Stepwise regression analysis showed that the variations of gross N mineralization rate was best described by the microbial biomass C and N.