Abstract
ABSTRACT A method for extracting DNA from laminarialean algae resulting in DNA of sufficient quantity and purity for DNA fingerprints is presented. The method both eliminates the use of liquid N2 and delays the use of toxic chemicals in the initial extraction steps; thus, it is appropriate for use in remote field locations. The algal samples were ground in a solution of hexadecyltrimethylammonium bromide (CTAB) and polyvinylpyrrolidone (PVPP) at room temperature and then stored for 1 week in the CTAB‐PVPP solution at room temperature before extraction with chloroform‐isoamyl alcohol and purification by cesium chloride ultracentrifugation. No degradation of DNA was observed. Hybridization of RsaI‐digested Macrocystis pyrifera (L.) C. Ag. DNA with the M13repeat probe yielded a DNA fingerprint with 12 discrete bands 4–19 kb in molecular size.
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