Abstract
Fibrinogen-related proteins (FREPs) play a crucial role in invertebrate immune response. In this study, we acquired a novel fibrinogen-related protein gene in Litopenaeus vannamei coding for one kind of fibrinogen-related protein, designated as LvFREP2. The complete cDNA sequence of LvFREP2 was 1903 bp long, containing an open reading frame of 1479 bp coding for LvFREP2. The LvFREP2 protein contained a putative signal peptide and a fibrinogen-related protein domain. qRT-PCRs indicated that LvFREP2 mRNA ubiquitously distributed in all examined tissues, and it was up-regulated in gills after V. harveyi and LPS challenges. The recombinant LvFREP2 agglutinated Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Vibrio alginolyticus, V. cholerae, V. vulnificus, V. parahaemolyticus, V. harveyi, Pseudomonas aeruginosa, P. fluorescens) in a calcium-dependent manner. LvFREP2 also facilitated the clearance of Vibrio harveyi in vivo. Therefore, our results suggested that lvFREP2 may have important roles in the anti-bacterial immunity of L. vannamei.
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