Abstract

We develop a fast fiber-based in situ fluorescence system for phytoplankton taxonomy. The fluorescence is excited by high power LEDs with different wavelengths through a customized fiber bundle to the place of interest. Sinusoidal-amplitude-modulation (SAM) techniques are employed. The detection time is shorten to 0.1s because the fluorescence is excited and demodulated by different wavelengths simultaneously. The fluorescence signal is demodulated by a digital lock-in amplifier. The device is tested in Shenzhen Bay, South China Sea.

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