Abstract
The Feulgen and Rossenbeck staining procedure reveals in all embryonic and adult cell types of the quail ( Coturnix coturnix japonica) one or several chromatin condensations in the interphase nucleus. The Unna-Pappenheim technique, combined with RNAase treatment according to Brachet, shows that these chromatin masses are associated with the nucleolar RNA. Electron microscopic studies confirm this observation and the EDTA preferential staining procedure for RNP according to Bernhard makes it possible to distinguish three main types of nucleoli in the various tissues of the quail showing different patterns of RNA and DNA relationships. The functional significance of the large amount of nucleolus-associated chromatin in the quail, and of the more or less intimate relationships between RNA and DNA in the various types of nucleoli are discussed.
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