A Ferrofluid with Surface Modified Nanoparticles for Magnetic Hyperthermia and High ROS Production.

Oscar Cervantes,Norberto Casillas,Luis Hector Quintero,Nayeli Checa,Mario Eduardo Cano,Peter Knauth,Zaira Del Rocio Lopez,Francisco Apolinar Cholico,Rodolfo Hernandez-Gutiérrez,Jose Avila Paz

doi:10.3390/molecules27020544

Abstract

A ferrofluid with 1,2-Benzenediol-coated iron oxide nanoparticles was synthesized and physicochemically analyzed. This colloidal system was prepared following the typical co-precipitation method, and superparamagnetic nanoparticles of 13.5 nm average diameter, 34 emu/g of magnetic saturation, and 285 K of blocking temperature were obtained. Additionally, the zeta potential showed a suitable colloidal stability for cancer therapy assays and the magneto-calorimetric trails determined a high power absorption density. In addition, the oxidative capability of the ferrofluid was corroborated by performing the Fenton reaction with methylene blue (MB) dissolved in water, where the ferrofluid was suitable for producing reactive oxygen species (ROS), and surprisingly a strong degradation of MB was also observed when it was combined with H2O2. The intracellular ROS production was qualitatively corroborated using the HT-29 human cell line, by detecting the fluorescent rise induced in 2,7-dichlorofluorescein diacetate. In other experiments, cell metabolic activity was measured, and no toxicity was observed, even with concentrations of up to 4 mg/mL of magnetic nanoparticles (MNPs). When the cells were treated with magnetic hyperthermia, 80% of cells were dead at 43 °C using 3 mg/mL of MNPs and applying a magnetic field of 530 kHz with 20 kA/m amplitude.

Highlights

IntroductionThe stable surface binding between iron oxide nanoparticles and catechol (or 1,2benzenediol) derived compounds is well documented [1]
The stable surface binding between iron oxide nanoparticles and catechol derived compounds is well documented [1]
At room temperature, reactive oxygen species (ROS) production is regulated when magnetic nanoparticles (MNPs) are coated with different organic compounds when compared with uncoated MNPs [18]. These results suggest that the ROS production using iron oxide MNPs can be modulated through their surface modification

Summary

Introduction

The stable surface binding between iron oxide nanoparticles and catechol (or 1,2benzenediol) derived compounds is well documented [1]. In colloidal suspensions, their long-time stability has been studied [2], as well as their good molecular anchoring properties [1,2,3]. The excessive production of ROS represents a serious risk factor for producing other diseases such as cancer [11] or Parkinson’s disease [12] When this overproduction of ROS occurs in a living system, the internalization of antioxidant substances is the ideal mechanism to reach the normal production rate [13]

Objectives

Results

Conclusion