Abstract

A feeding preference bioassay was developed that provides consistent qualitative and quantitative evaluation of antifeedant activity of a candidate compound to larvae of several Neodiprion sawflies. The bioassay utilizes twigs of mature jack pine, Pinus banksiana, foliage; one-half is treated with the antifeedant deet and one-half nontreated. Neodiprion sawflies exhibit natural behavioral characteristics in this bioassay, and upon detection of the antifeedant they migrate to untreated foliage to feed.

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