Abstract
Background Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis. Accurately, quantifying the tumor vasculature can help understanding CSC characteristics and improve cancer diagnosis, therapy planning, and evaluation. The objective of this study is to present a method for improved angiogenesis assessment. Methods We proposed a three-dimensional microvessel density (3D MVD) to evaluate tumor angiogenesis and tested it in animal models. Six male Balb/c nude mice were divided into normal group and tumor group. The mice in tumor group were orthotopically implanted human gastric cancer, cell line BGC-823. The phase-contrast images were collected at Shanghai Synchrotron Radiation Facility BL13W beamline, which has much higher soft-tissue contrast and spatial resolution than conventional X-ray. After volume reconstruction and vessel extraction, the 3D models of the angiogenesis were established for MVD calculation. Results The results showed that the proposed 3D MVD is positively correlated with the pathological changes of the microvessels. It took the advantage of high resolution of the phase-contrast imaging and added three-dimensional information to the existing MVD measure. Conclusions Our study presents a feasible approach for better understanding of tumor angiogenesis. It may provide doctors and scientists a better tool for cancer investigation and improving medical outcomes.
Highlights
Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis
0 Surface Inside (c) images were taken during the Phase-contrast computed tomography (PC-CT) experiment (Figure 2(a))
These volume of interest (VOI) have the same size of 100 × 100 × 100 pixels
Summary
Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis. Quantifying the tumor vasculature can help understanding CSC characteristics and improve cancer diagnosis, therapy planning, and evaluation. The results showed that the proposed 3D MVD is positively correlated with the pathological changes of the microvessels It took the advantage of high resolution of the phase-contrast imaging and added threedimensional information to the existing MVD measure. Our study presents a feasible approach for better understanding of tumor angiogenesis It may provide doctors and scientists a better tool for cancer investigation and improving medical outcomes. A quantitative measure of angiogenesis can provide insight for CSC characteristics as well as play a pivotal role in cancer diagnosis, therapy planning, and evaluation. How to accurately quantify tumor vasculature still remains a challenging task
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