Abstract
A continuous-flow mixing device was developed for fast concentration changes in a 0.25 μt volume. The mixing chamber is designed to study the effects of fast concentration changes in the bathing solution on small probes (e.g. chemoreceptive hairs or membrane patches). It consists of a freely accessible hanging droplet which is viewed with a microscope. Mixtures are generated by injecting variable amounts of a fluid into another fluid under photometric control. Time courses for the concentration steps are (computer-)generated as voltage-time courses which serve as set point of the injection-control servo circuit. Positive steps of the concentration of the injected fluid can be reached within less than 30 ms; falling concentrations are determined by wash-out time constants around 100 ms.
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