Abstract
A method for the rapid analysis of the 5′ ends of deoxyribooligonucleotides is described. The method involves: (a) labeling of the 5′ ends with polynucleotide kinase; (b) digestion of the oligonucleotides to 5′ mononucleotides with pancreatic DNAase and snake venom exonuclease; (c) unidimensional separation on polyethyleneimine plates of the four 5′ mononucleotides, ATP, and inorganic phosphate. This method requires only a minimum amount of work, time, and material.
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