Abstract
Abstract A simple procedure is described for co-immobilization of glucose oxidase and dimethylaminomethylferrocene in a sodium alginate gel on the surface of pyrolytic graphite electrode. The film is electrochemically active and the peak current is a function of D-glucose concentration with the apparent Michaelis-Menten constant Km = (1.2±0.3) × 10−2 M. The optimal concentration range for biosensoric applications in 0.001–0.010 M. §On leave from the Institute of Applied Enzymology FERMENTAS, Vilnius, Lithuanian Republic.
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