Abstract
In this study, a new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. The quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. The quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. Chromatographic separation was achieved using a C18 column (150 mm × 4.6 mm, 5.0 μm), and the mobile phase was composed of acetonitrile-0.05 mol·L−1 potassium dihydrogen phosphate (pH 4.0) (25 : 75) at a flow rate of 1.0 mL·min−1 and detection wavelengths of 307 nm (ondansetron, azasetron, ramosetron), 302 nm (granisetron), and 285 nm (tropisetron). In addition, the accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. The established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals.
Highlights
A new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. e quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. e quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors
The accuracy of the quantitative analysis of multicomponents by a single marker method was compared with an external standard method, and no significant difference was observed between the two methods. e established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals
There are no reports concentrating the QAMS or external standard method (ESM) method on the simultaneous quantification of ODT, GNT, TPT, AZT, and RMT in infusion samples and in injection dosage form. us, the purpose of this study was to establish a rapid HPLC-QAMS method for the detection of five 5-HT3 receptor antagonists by using two indexes of relative retention time and UV spectrum similarity for qualitative analysis and a relative correction factor for quantitative analysis. is method was successfully employed for the routine quality control of 5-HT3 receptor antagonist injection, infusion products, and the preliminary screening of unknown drugs in hospitals
Summary
A new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. e quantitative analysis of multicomponents by a single marker method was conducted with ondansetron as an internal reference substance and performed using relative retention time and ultraviolet spectral similarity as the double indicator. e quantitative analysis of the 5-HT3 receptor antagonists was calculated and investigated based on the relative correction factors. A new strategy for the simultaneous quantization of five serotonin 5-hydroxytryptamine receptor antagonists—ondansetron, azasetron, ramosetron, granisetron, and tropisetron—either in infusion samples or in injection dosage form was first established based on high-performance liquid chromatography combined with a quantitative analysis of multiple components by a single marker. E established method is rapid, is easy, and does not require many reference substances, and it can been successfully applied as part of the quality control of the five 5-HT3 receptor antagonists in their injection dosage form and infusion sample drugs in hospitals. Us, the purpose of this study was to establish a rapid HPLC-QAMS method for the detection of five 5-HT3 receptor antagonists by using two indexes of relative retention time and UV spectrum similarity for qualitative analysis and a relative correction factor for quantitative analysis. There are no reports concentrating the QAMS or ESM method on the simultaneous quantification of ODT, GNT, TPT, AZT, and RMT in infusion samples and in injection dosage form. us, the purpose of this study was to establish a rapid HPLC-QAMS method for the detection of five 5-HT3 receptor antagonists by using two indexes of relative retention time and UV spectrum similarity for qualitative analysis and a relative correction factor for quantitative analysis. is method was successfully employed for the routine quality control of 5-HT3 receptor antagonist injection, infusion products, and the preliminary screening of unknown drugs in hospitals
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