A fast and accurate method for genotyping the angiotensin-converting enzyme I/D polymorphism.

Abstract

To the Editor: The association of the insertion/deletion (I/D) polymorphism of the ACE gene with ischemic vascular disease is disputed.1 Mistyping as DD of 4% to 5% of ID subjects may contribute to conflicting findings.2 3 The original method4 preferentially extends allele D. The addition of 5% DMSO, or the use of an allele-specific oligonucleotide (ASO), reduces mistyping.5 6 Although the ASO technique is the method of choice,2 3 5 it is time consuming because a second nested PCR must be performed on the DD samples obtained with the original method. We have devised a one-step multiplex PCR, which we tested on 520 individuals and compared the results with …