Abstract
An efficient and high yield procedure for the synthesis of the difluoromethylene analog of β-aspartyl phosphate, β-aspartyl difluoromethylenephosphonate (βAFP), is described. Preincubation of βAFP with L-aspartate-β-semialdehyde dehydrogenases from several infectious microorganisms leads to potent time-dependent enzyme inactivation.
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