Abstract
Nanospheres have found versatile applications in the biomedical field; however, their possible harmful effects on immune and inflammatory systems are also a crucial concern. Inspired by a pomegranate structure, we demonstrated a novel structure for the nanostructured microspheres to overcome the drawbacks of nanospheres without compromising their merits. In this study, 3D pomegranate-like polydopamine microspheres (PDAMS) were synthesized by self-oxidative polymerization of dopamine hydrochloride. Herein, controlling the pH during polymerization led to synthesizing homogeneous agglomerated nano-sized spheres (400–2000 nm) and finally forming tunable and monodisperse micron-sized particles (21 µm) with uniform spherical shape porous microstructure. PDAMS interaction with the potential targets, Bone morphogenetic protein-2 (BMP2), Decorin, and Matrilin-1, was investigated via molecular calculations. Theoretical energy analysis revealed that PDAMS interaction with BMP2, Decorin, and Matrilin-1 is spontaneous, so that a protein layer formation on the PDAMS surface suggests application in bone and cartilage repair. It was also observed that PDAMS presented in-vitro degradation within 4 weeks. Here, disappearance of the UV-VIS spectrum peak at 280 nm is accompanied by the degradation of catechol groups. Pomegranate-like PDAMS support the biomimetic formation of hydroxyapatite-like layers, making them appropriate candidates for hard tissue applications. Herein, the appearance of peaks in XRD spectrum at 31.37, 39.57, 45.21, and 50.13° attributed to hydroxyapatite-like layers formation. All these results demonstrated that self-oxidative polymerization under a controllable pH can be a green and straightforward technique for preparing the pomegranate-like PDAMS and providing an innovative basis for further pre-clinical and clinical investigations.
Highlights
Nanospheres are abundantly used in the biomedical area due to their superior properties
The particles were synthesized under self-oxidative polymerization of dopa-HCl in pH 8.5, which was controlled to be stable within 3 days of synthesis
The pH was controlled and fixed to 8.5 during the polymerization process that was led to the homogeneous agglomeration of nano-sized spheres and formation of tunable and monodisperse micron-sized particles with uniform spherical shape and porous microstructure
Summary
Reviewed by: Baolin Guo, Xi’an Jiaotong University, China Payam Zarrintaj, University of Montana, United States Lulu Han, Dalian University of Technology, China. 3D pomegranate-like polydopamine microspheres (PDAMS) were synthesized by self-oxidative polymerization of dopamine hydrochloride. Theoretical energy analysis revealed that PDAMS interaction with BMP2, Decorin, and Matrilin-1 is spontaneous, so that a protein layer formation on the PDAMS surface suggests application in bone and cartilage repair. Pomegranate-like PDAMS support the biomimetic formation of hydroxyapatite-like layers, making them appropriate candidates for hard tissue applications. The appearance of peaks in XRD spectrum at 31.37, 39.57, 45.21, and 50.13° attributed to hydroxyapatite-like layers formation. All these results demonstrated that self-oxidative polymerization under a controllable pH can be a green and straightforward technique for preparing the pomegranate-like PDAMS and providing an innovative basis for further preclinical and clinical investigations
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.