Abstract
Glutathione (GSH) provides essential functions towards metabolism process in biological systems and is related to many diseases such as human immunodeficiency virus, Parkinson, cardiovascular diseases, etc. In this work, a very simple, fast and label-free colorimetric method for highly sensitive GSH quantification is proposed. This strategy is based on the color change of MnO2 nanosheets solution from yellowish-brown to colorless. Without GSH, MnO2 nanosheets exhibit a strong ultraviolet–visible (UV–vis) absorption peak at 376nm with the color showing as yellowish-brown. Once GSH is introduced, MnO2 nanosheets are reduced to Mn2+ ions rapidly, which leads to a yellowish-brown color fading and a remarkable decrease of the absorbance at 376nm. Based on the distinct color change, a method for highly sensitive and selective detection of GSH was realized with bare eyes and UV–vis spectroscopy, respectively. This method has a wide dynamic range from 0.5μM to 100μΜ and shows a good linear relationship from 0.5μM to 10μΜ with a detection limit of 0.1μM. More importantly, this procedure is very simple and the whole assay can be accomplished quickly within 5min. Additionally, this developed assay has also been successfully applied to the detection of GSH in biological fluids with satisfactory results.
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