Abstract
Abstract THIS PAPER DESCRIBES DEVELOPMENT OF A QUANTITATIVE HPTLC ASSAY FOR DETERMINATION OF HISTAMINE RELEASED FROM THE RAT SEROSAL MAST CELLS. BRIEFLY, HISTAMINE IS QUANTIFIED BY DERIVATIZATION WITH DANSYL CHLORIDE, THEN SEPARATED BY HPTLC AND DETECTED BY DENSITOMETRY SCANNING UNDER FLUOROMETRIC MODE. THE METHOD USES R-PHENYLEPHRINE AS AN INTERNAL STANDARD, AND SHOWS AN EXCELLENT LINEARITY OVER THE RANGE OF 50 TO 300 NG/ML THE CHROMATOGRAPHIC CONDITIONS ALLOW RESOLUTION OF THE DANSYLATED HISTAMINE AND INTERNAL STANDARD FROM THE OTHER DANSYLATED CO-EXTRACTABLE POLYAMINES.
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