Abstract
Normal 0 7.8 磅 0 2 false false false EN-US ZH-CN X-NONE MicrosoftInternetExplorer4 The junction of nanotechnology and biomedical sciences unfasten the possibility for a wide range of biological research topics and medical uses at the molecular and cellular level. In the present study silver nanoparticles were synthesized by using Leucas aspera leaf extract support of biological process. The extract incubated with 1mM AgNO 3 solution showed gradual change in the colour of the extract from greenish to reddish brown. Reduction of silver ions by the leaf extract was examined by UV-visible spectroscopic technique. Further, it was confirmed by Fourier Transformations Infrared spectroscopic (FTIR) analysis. The purity of the synthesized silver nanoparticles was examined through X-Ray Diffraction (XRD) analysis. The nanoparticles were examined for the antibacterial activities of antibiotic (Tetracycline), silver nanoparticles and the combined effect of both antibiotic and nanoparticles against the multi drug resistant human pathogens .
Highlights
Green chemistry is intended for the implementation of chemical products and processes to reduce or eliminate the use and generation of substances hazardous to human health and environment in the synthesis of metal nanoparticle by the reduction of the corresponding metal ion salt solutions
The antibacterial effect of silver nanoparticles of Leucas aspera was evaluated against four pathogenic bacteria, including Vibrio cholerae, Klebsiella pneumoniae (Gram negative) and Staphylococcus aureus, Micrococcus luteus (Gram positive) using the agar well diffusion method
The bacterial strains Staphylococcus aureus, Vibrio cholerae, Micrococcus luteus and Klebsiella pneumoniae were collected from the Microbial Type Culture Collection (MTCC), The Institute of microbial Technology, Chandigarh, India
Summary
Green chemistry is intended for the implementation of chemical products and processes to reduce or eliminate the use and generation of substances hazardous to human health and environment in the synthesis of metal nanoparticle by the reduction of the corresponding metal ion salt solutions. The synthesis and characterization of silver nanoparticles were synthesized by using Leucas aspera leaf extract support of biological process. Ag-NPs were prepared using silver nitrate as silver precursor and extract of Leucas aspera leaf as reducing agent and stabilizer. The antibacterial effect of silver nanoparticles of Leucas aspera was evaluated against four pathogenic bacteria, including Vibrio cholerae, Klebsiella pneumoniae (Gram negative) and Staphylococcus aureus, Micrococcus luteus (Gram positive) using the agar well diffusion method
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