Abstract
In this study, the potentiometric arrayed glucose biosensors, which were based on zinc oxide (ZnO) or aluminum-doped zinc oxide (AZO) sensing membranes, were fabricated by using screen-printing technology and a sputtering system, and graphene oxide (GO) and Nafion-glucose oxidase (GOx) were used to modify sensing membranes by using the drop-coating method. Next, the material properties were characterized by using a Raman spectrometer, a field-emission scanning electron microscope (FE-SEM), and a scanning probe microscope (SPM). The sensing characteristics of the glucose biosensors were measured by using the voltage–time (V-T) measurement system. Finally, electrochemical impedance spectroscopy (EIS) was conducted to analyze their charge transfer abilities. The results indicated that the average sensitivity of the glucose biosensor based on Nafion-GOx/GO/AZO was apparently higher than that of the glucose biosensor based on Nafion-GOx/GO/ZnO. In addition, the glucose biosensor based on Nafion-GOx/GO/AZO exhibited an excellent average sensitivity of 15.44 mV/mM and linearity of 0.997 over a narrow range of glucose concentration range, a response time of 26 s, a limit of detection (LOD) of 1.89 mM, and good reproducibility. In terms of the reversibility and stability, the hysteresis voltages (VH) were 3.96 mV and 2.42 mV. Additionally, the glucose biosensor also showed good anti-inference ability and reproducibility. According to these results, it is demonstrated that AZO is a promising material, which could be used to develop a reliable, simple, and low-cost potentiometric glucose biosensor.
Highlights
Glucose plays a vital role in numerous physiological processes, and all cells and organs in the human body require glucose as a source of energy in order to function properly [1]
glucose oxidase (GOx)/graphene oxide (GO)/aluminum-doped zinc oxide (AZO) performed well, with excellent average sensitivity (15.44 mV/mM), which was markedly higher than that of the glucose biosensor based on Nafion-GOx/GO/zinc oxide (ZnO) (11.92 mV/mM)
We proposed a facile method for the development of the potentiometric arrayed glucose biosensor based on a PET substrate
Summary
Glucose plays a vital role in numerous physiological processes, and all cells and organs in the human body require glucose as a source of energy in order to function properly [1]. Developed a potentiometric glucose biosensor by immobilizing GOx onto a ZnO nanowires (NWs)/Ag electrode. They compared the addition of bovine serum albumin (BSA) regarding the growth of. We applied AZO to the sensing membranes of potentiometric arrayed glucose biosensors, thereby analyzing whether AZO was more suitable for biosensing than ZnO. We proposed a potentiometric arrayed glucose biosensor based on a terephthalate (PET) substrate, which has many advantages, such as its portability, flexibility, miniaturization of sensors, and low cost [29]. We investigated the sensing characteristics of the potentiometric arrayed glucose biosensor based on Nafion-GOx/GO/AZO, which included the average sensitivity, linearity, LOD, response time, reversibility, selectivity, and reproducibility. The performances of the proposed glucose biosensor were compared with potentiometric glucose biosensors that have been developed in recent years
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