Abstract
In this paper, a facile and sensitive biocompatible biosensor based on Nafion/choline oxidase/manganese dioxide composite film was developed for the determination of choline chloride. Manganese dioxide (MnO2) nanoparticles, possessing the advantages of large specific surface areas, good hydrophilicity, great permeability as well as excellent biocompatibility, were synthesized in-situ at the surface of the glassy carbon electrode (GCE) by one-step electrodeposition. And then, choline oxidase (ChOx) was immobilized on the MnO2 modified GCE with coating a Nafion film to hold the ChOx/MnO2 film on the electrode surface firmly. Upon optimized conditions, a linear range of 8.0–1.0mM was obtained for the sensor in a cyclic voltammetry method, with a detection limit as low as 5.0µM. Besides, the biosensor was successfully employed to detect choline in milk, milk powder and feedstuff samples, providing a promising alternative for the practical application.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
