Abstract
Hydrogen sulfide (H2S) is a gaseous signaling transmitter which plays a crucial role in cell fates. Given the volatility of H2S in live cells corresponding to the various cell activities, it is important to design and synthesize novel fluorescent probes which could enter cells rapidly for the fast H2S detection. Herein, we reported a turn-on fluorescent probe P1 for H2S labeling in live cells, based on the dynamic thiol-disulfide exchange mechanism on cell surface, which facilitated the probe to enter cells quickly. High performance liquid chromatography (HPLC) results indicated the decoration of cell-penetrating disulfides endowed P1 with a high cellular uptake efficiency. Fluorescence microscopic imaging for HeLa cells suggested P1 was competent for intracellular H2S detection at submicromolar level. We expected our simple but effect strategy which enhanced the cell permeability of probes to benefit more accurate detection for intracellular specific molecule.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
