Abstract
BackgroundRetinol binding protein 4 (RBP4) has been regarded as an important serological biomarker for type 2 diabetes mellitus (T2DM). Hence, the construction of a highly sensitive detection method for RBP4 is the key to early prevention and multidisciplinary intervention of T2DM. In this work, a dual-quenched electrochemiluminescence (ECL) immunosensor has been fabricated for ultrasensitive detection of RBP4 by combining zeolitic imidazolate framework-67/AuPt-supported luminol (luminol@AuPt/ZIF-67) with MnO2 nanosheets-grown on carbon nanotubes (MnO2@CNTs).ResultsAuPt/ZIF-67 hybrids with high-efficiency peroxidase-like activity could provide multipoint binding sites for luminol and antibodies and significantly boost the amplified initial signal of the ECL immunosensor. Upon glutathione/H2O2 coreactants system, MnO2@CNTs composites could quench the initial signal by inhibiting mimic peroxidase activity of luminol@AuPt/ZIF-67. Moreover, the absorption spectrum of the MnO2@CNTs composites completely overlaps with the emission spectrum of luminol, which can further reduce initial signal by ECL resonance energy transfer (ECL-RET).ConclusionsBenefiting from the above-mentioned properties, the designed immunoassay sensitivity exhibited excellent sensitivity and relative stability for RBP4 detection range from 0.0001 to 100 ng mL−1 with a low detection limit of 43 fg mL−1. Therefore, our ECL immunosensor provides an alternative assaying strategy for early diagnosis of T2DM.Graphic abstract
Highlights
Retinol binding protein 4 (RBP4) has been regarded as an important serological biomarker for type 2 diabetes mellitus (T2DM)
Characterization of Zeolitic imidazolate frameworks (ZIFs)‐67 and AuPt/ZIF67 The size, morphology and elemental composition of the as-synthesized nanomaterials were characterized by Transmission electron microscopy (TEM) and Scanning transmission electron microscopy-energy dispersive X-ray spectroscopy (STEM-energy-dispersive X-ray spectroscopy (EDS))
After decorating with AuPt NPs, it was clear that the AuPt NPs were homogeneously dispersed on the surface of ZIF-67 (Fig. 1C, D) with an apex-to-apex diameter about 3 nm
Summary
Retinol binding protein 4 (RBP4) has been regarded as an important serological biomarker for type 2 diabetes mellitus (T2DM). The construction of a highly sensitive detection method for RBP4 is the key to early prevention and multidisciplinary intervention of T2DM. According to Global Report on Diabetes of WHO in 2016 and The Berlin Declaration in 2018, early detection and proper multidisciplinary interventions are. Retinol binding protein 4 (RBP4), an insulin resistance-related hormone, is a potential serological biomarker for early monitoring and diagnosis of T2DM [5,6,7]. Zeolitic imidazolate frameworks (ZIFs), an attractive subclass of MOFs, have received considerable attention in the bio-analysis applications ascribing to their unique characteristics of large specific surface area, adjustable structure and ordered crystalline pores [14]. The imidazolate linkers, especially the imidazole nitrogen site within the porous frameworks, endow ZIFs with excellent selective adsorption properties [15] and catalytic activities [16], making them good candidates in the field of electrochemical sensing
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