Abstract
A dual-mode fluorescent probe (Probe 1) was developed for the separate detection of Hg2+ and H2S. Probe 1 could detect Hg2+ and H2S through two different reaction sites, with the mechanism of Probe 1 for Hg2+ depending on the desulfurization reaction and for H2S depending on the nucleophilic addition reaction of an aldehyde with H2S. Separate detection of Hg2+ and H2S was achieved by determining the fluorescence emission wavelength of Probe 1-Hg2+ (443 nm, λab = 247 nm) and Probe 1-H2S (504 nm, λab = 256 nm). The fluorescence intensity of Probe 1 has a linear relationship with the concentration of Hg2+ in the range 0–7 μM and the LOD was 39.28 nM. And H2S in the range 0–110 μM, the LOD was 0.7449 μM. With increasing concentrations of Hg2+, test strips gradually became darker in color. The luminescence intensity of test strips gradually increased with increasing H2S concentration, and the initially colorless Probe 1 solution gradually changed to yellow under natural light with increasing H2S concentration. Probe 1 could be used for Hg2+ and H2S detection in real samples.
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