Abstract
Bifunctional fluorescent probe has excited extensive interest in view of its dual-emission detection mode, favorable efficiency and low cost. Herein, a novel coumarin-based dual responsive fluorescent probe NNH was synthesized for response to Cu2+ and H2S with different fluorescence signals. This probe displayed a significant “turn-off” fluorescence response to Cu2+, and the limit of detection (LOD) for Cu2+ was calculated to be 0.356 µM. On the other hand, NNH exhibited a ratiometric fluorescence response to H2S with highly sensitivity (detection limit 4.266 µM), and the fluorescence color change from orange to cyan in the presence of H2S. The sensing mechanisms of NNH between Cu2+ and H2S were approved by Job's plot, ESI-MS analysis, and density functional theory (DFT) calculations. Besides, NNH could be served as a sensing tool for Cu2+ and H2S in environmental samples and living zebrafish.
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