Abstract
In this study, a dual-task method for the simultaneous detoxification and enrichment of the pharmacologically active compound, 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glycoside (TSG) from crude extract of Polygonum multiflorum roots was established by macroporous resin. The performances of TSG on six macroporous resins were compared with regard to their adsorption and desorption properties. Kinetics tests indicated that the adsorption process fitted the pseudo-second-order model much better than the pseudo-first-order model. The solute affinity of TSG towards the AB-8 resin at different temperatures was described in terms of Langmuir and Freundlich isotherms. The optimal adsorption conditions were: 2.24mg/mL of TSG in feed solution, 4.5bedvolume (BV) of loading volume, loading time no less than 120min. The elution conditions were as follows: 6.0BV of water, followed by 2.5BV of 40% ethanol with flow rate of 1.5BV/h. After the AB-8 resin treatment, the concomitant hepatotoxic anthraquinones were almost completely removed. Meanwhile, the purity of TSG was 8.6-fold increased from 7.51% to 64.76% with a recovery of 68.34%. The process fulfilled the task of detoxification and enrichment of TSG, and proved to be a promising basis for pharmaceutical preparations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.