A dual-signal fluorometric-colorimetric sensing platform and visual detection with a smartphone for the determination of β-galactosidase activity based on fluorescence silicon nanoparticles.

Abstract

As one of primary biomarkers of ovarian cancer in early stages, β-galactosidase (β-Gal) is significant in the discovery and diagnosis of the disease. In this work, we constructed a multi-signal sensing platform based on silicon nanoparticles (Si NPs) for β-Gal activity determination. When β-Gal was introduced to the sensing system, 2-Nitrophenyl β-D-galactopyranoside (ONPG) could be converted to o-Nitrophenol (o-NP), which had a characteristic absorption peak at 416nm and the colorless solution turned yellow. The fluorescence emission of Si NPs at 450nm can be greatly quenched by o-NP as a consequence of inner filter effect (IFE). This dual-signal fluorometric and colorimetric determination approach could be utilized to detect β-Gal in the range of 2-120 U/L and 6-120 U/L. The limits of detection were 1.36 U/L and 1.07 U/L, respectively. This sensing platform could be successfully utilized to detect β-Gal in real samples. Additionally, a visual detection method was designed to achieve quantitative analysis of β-Gal with the assistance of the smartphone.