Abstract
Real-time monitoring of dissolved oxygen (DO) and pH is of great significance for understanding cellular metabolism. Herein, a dual optical pH/O2 sensing membrane was prepared by the electrospinning method. Cellulose acetate (CA) and poly(ε-caprolactone) (PCL) nanofiber membrane blended with platinum (II)-5,10,15,20-tetrakis-(2,3,4,5,6-pentafluorophenyl)-porphyrin (PtTFPP) was used as the DO sensing matrix, upon which electrospun nanofiber membrane of chitosan (CS) coupled with fluorescein 5-isothiocyanate (FITC) was used as the pH sensing matrix. The electrospun sensing film prepared from biocompatible biomaterials presented good response to a wide range of DO concentrations and physiological pH. We used it to monitor the exracellular acidification and oxygen consumption levels of cells and bacteria. This sensing film can provide a luminescence signal change as the DO and pH change in the growth microenvironment. Due to its advantages of good biocompatibility and high stability, we believe that the dual functional film has a high value in the field of biotechnology research.
Highlights
The monitoring of metabolite parameters associated with cellular growth is essential for biological and clinical studies [1]
During the processes of cellular respiration and metabolism, the consumption of oxygen and the production of metabolites will lead to a change in the concentration of dissolved oxygen (DO) and pH level in the culture microenvironment [2–5]
Sensors for real-time monitoring of DO and pH in the cellular growth microenvironment are of great significance
Summary
The monitoring of metabolite parameters associated with cellular growth is essential for biological and clinical studies [1]. Optical sensors based on reversible luminescence changes in specific indicators show high potential for environmental detection, due to the advantages of fast, simple and easyto-read signals [6]. These sensors composed with probes and polymer matrices can clearly reflect the environmental changes through luminescence variations. Biodegradable poly(ε-caprolactone) (PCL) was blended with CA as an oxygen-sensitive matrix for obtaining electrospun DO sensor films. The functionalized films were used to monitor cellular respiration and metabolism, demonstrating the real-time biomonitoring capability of the sensing films
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