A dual-mode ratiometric fluorescence and smartphone-assisted colorimetric sensing platform based on bifunctional Fe,Co-CQD for glucose analysis at physiological pH

Abstract

Diabetes is a common and fatal chronic metabolic disease characterized by hyperglycemia, and thus monitoring blood glucose level is essential for early screening and timely control of disease. Herein, we have prepared the bifunctional iron and cobalt co-doped carbon quantum dot (Fe,Co-CQD) with good optical properties and peroxidase-mimetic catalytic activity toward specific substrate o-phenylenediamine (OPD) under alkaline condition. Glucose oxidase (GOx) specifically catalyzes the oxidation of glucose into H2O2, and Fe,Co-CQD subsequently triggers a reaction between H2O2 and OPD to form yellow product DAP with a distinct UV absorption peak at 420 nm. Simultaneously, the generated DAP also appears a well-defined fluorescence signal at 555 nm, which can suppress the intrinsic fluorescence peak of Fe,Co-CQD (439 nm) owing to the inner filter effect (IFE). Based on this principle, a dual-mode ratiometric fluorescence and colorimetric sensing platform has been constructed for glucose analysis at physiological pH, which reveals the advantages of excellent accuracy, high throughput, simple operation, and low cost. More importantly, a smartphone-assisted colorimetric sensing system based on a portable visual detection kit and a 3D printing smartphone-based device has been constructed, which enables on-site detection of glucose in complex serum samples without laboratory instruments, indicating its potential practical application prospect.