Abstract
A dual-label time-resolved fluorescence immunoassay (TRFIA) for the simultaneous determination of β human chorionic gonadotropin and progesterone was developed for the early diagnosis of ectopic pregnancy. The performance of this assay was evaluated using clinical serum and then compared with standard procedures. The sensitivity for β human chorionic gonadotropin detection was 1 U/L (linear dynamic range, 0–8000 U/L), and the sensitivity for progesterone detection was 0.05 ng/mL (linear dynamic range, 0–50 ng/mL). High correlation coefficients (R2) were obtained between the reported immunoassay and standard methods (R2 = 0.99 for β human chorionic gonadotropin, R2 = 0.97 for progesterone). The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical analyses and is a suitable alternative to the single-labeled diagnostic methods.
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