Abstract
A novel microfluidic paper-based analytic device (μPAD) biosensor is developed for sensitive and visualized detection of glucose. This biosensor is easily fabricated using the wax printing technique, with a hybrid nanocomplex composed of dual enzymes glucose oxidase (GOx) and horseradish peroxidase (HRP) and Cu3(PO4)2 inorganic nanocrystals incorporated in the detection zones. The hybrid nanocomplex is found to exhibit a flower-like structure, which allows co-immobilization of these two enzymes in a biocompatible environment. These nanoflowers not only preserve the activity and enhance the stability of the enzymes, but also facilitate the transport of the substrates between the two enzymes. The biosensor is demonstrated to enable rapid and sensitive quantification of glucose in the concentration range of 0.1-10 mM with a limit of detection (LOD) of 25 μM. It is also shown to be applicable to colorimetric quantitative detection of glucose in human serum and whole blood samples, implying its potential for clinical applications.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
