Abstract
A dual column system for the determination of free amino acids in plant extracts is described. Both columns use Beckman AA-20 resin. All five buffers used are lithium citrate and vary according to pH and ionic strength. The acidic and neutral amino acids are eluted on a long column (resin height 37cm) with two buffers while the basic amino acids are eluted on a short column (resin height 19cm) with the remaining three buffers. The dual column method saves about 1.7 h per analysis when compared with the standard single column method with lithium citrate buffers.
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Schedule a callMore From: Canadian Institute of Food Science and Technology Journal
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