Abstract
A dual column system for the determination of free amino acids in plant extracts is described. Both columns use Beckman AA-20 resin. All five buffers used are lithium citrate and vary according to pH and ionic strength. The acidic and neutral amino acids are eluted on a long column (resin height 37cm) with two buffers while the basic amino acids are eluted on a short column (resin height 19cm) with the remaining three buffers. The dual column method saves about 1.7 h per analysis when compared with the standard single column method with lithium citrate buffers.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Canadian Institute of Food Science and Technology Journal/Journal de L'Institut Canadien de Science et Technologie Alimentaire
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
