A dsRNA delivery system based on the rosin-modified polyethylene glycol and chitosan induces gene silencing and mortality in Nilaparvata lugens.

Abstract

RNA interference (RNAi) technology has been considered as a promising pest control strategy due to its species selectivity. One of the popular RNAs is exogenous double strand RNA (dsRNA). However, dsRNA is easily degraded by nucleases and is difficult to pass through the insect body walls, and these factors restrict the application of RNAi technology in pest management. Here, the brown planthopper (BPH, Nilaparvata lugens), a major hemipteran pest of rice in Asia countries was used as a model insect, and a dsRNA topical delivery system was constructed. The carrier part of the delivery system was composed of rosin-modified polyethylene glycol and chitosan, termed ROPE@C. When the N/P ratio was greater than 1:1.25, the dsRNA/ROPE@C complex encouraged full binding of the dsRNA. The gel electrophoresis results showed that ROPE@C improved the stability of dsRNA in the presence of nucleases in gut and lumen contents for at least 6 h and in the temperature range from 4 °C to 37 °C. The dsNlCHSA/ROPE@C/alkyl polyglycoside (APG) nano-formulation directly penetrated the body walls reaching hemocoel within 6h, and consequently, the relative expression of chitin synthetase A (CHSA) in BPH was reduced by 54.3% and the mortality rate was 65.8%. We developed an appropriate delivery method for dsRNA application in BPH, which is helpful for a large-scale application of RNAi pesticides. © 2022 Society of Chemical Industry.