A Drosophila P5B ‐Type ATPase is Required for Polyamine Transport

Abstract

In eukaryotes P‐type ATPases are represented by a superfamily of membrane ion and lipid transporters divided into five subclasses. Substrates for subclasses 1–4 have been identified, however little is known about the substrate specificity of the P‐type 5A and 5B transporters. Here we show that the Drosophila P5B‐type ATPase is required for polyamine transport. The gene encoding the Drosophila P5B‐type ATPase produces multiple protein isoforms. Using a combination of genetic mutational analysis and CRISPR‐Cas9 gene editing we show that some of these isoforms are required for polyamine transport and others are not. In addition, we show that the Drosophila P5A‐type ATPase is not required for polyamine transport indicating a different substrate specificity for this transporter subfamily. In summary, we provide evidence for the first time of substrate specificity for the P5B‐type ATPase subfamily of transporters.Support or Funding InformationUniversity of Central Florida, College of Sciences Seed AwardThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.