A double carbon dot system composed of N, Cl-doped carbon dots and N, Cu-doped carbon dots as peroxidase mimics and as fluorescent probes for the determination of hydroquinone by fluorescence.

Abstract

A fluorescence method isdescribed for the determination of hydroquinone based on the double carbon dot system as peroxide mimic enzymes and fluorescent probes. Deep eutectic solvent (DES)-based fluorescent carbon dots (N/Cl-CDs) and copper-doped carbon dots (N/Cu-CDs) were prepared by the hydrothermal method. Both carbon dots were characterized with transmission electron microscopy (TEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, X-ray photoelectron spectrometry (XPS), Fourier transform infrared (FT-IR) spectroscopy, and fluorescence spectroscopy. N/Cl-CDs displayed intrinsic peroxidase-like activity and were able to catalyze the oxidation of hydroquinone (H2Q) to p-benzoquinone (BQ) along with an intermediate. The intermediate (BQ) did quench the N/Cu-CD photoluminescence (PL) at 450nm usingan excitation wavelength of 347nm. Based on the results, a fluorescent platform isproposed for the determination of hydroquinone with a promising determination limit of 0.04μM (linear range, 1.0-75μM). The recoveries of spikedwater samples were in the range 89.5-105.1%, with relative standard deviations (RSDs) of 1.5-2.9%. This method was applied to determination ofH2Q in environmental water samples. Graphical abstract A fluorescence method was established for the determination of hydroquinone based on the double carbon dot system as peroxide-mimic enzymes and fluorescent probes. Chlorine-doped carbon dots (N/Cl-CDs) derived from deep eutectic solvent (DES) displayed intrinsic peroxidase-like activity, and were able to catalyze the oxidation of hydroquinone (H2Q) to p-benzoquinone (BQ) along with an intermediate. The intermediate (BQ) did quench the N/Cu-CD photoluminescence (PL). This method was applied to H2Q in environmental water samples.