Abstract
A reliable, convenient and economical radioimmunoassay (RIA) for serum progesterone has been established and tested. This procedure employs diethyl ether extraction followed by RIA utilizing rabbit anti-11α-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11α-BSA) serum, progesterone-3-(O-carboxymethyl)oximino-[ 125I]-iodohistamine (progesterone-3-[ 125I]) as radioligand and goat anti-rabbit gamma globulin as second antibody. In conjunction with antiprogesterone-11α-BSA serum, the overall assay specificity of the progesterone-3-[ 125I] ria is similar to that of the [ 3H]-progesterone method using dextran-coated charcoal. The results of serum progesterone measurements during the menstrual cycle obtained by the progesterone-3-[ 125I] RIA appear comparable to those of [ 3H]-progesterone assays which employ similar anti-progesterone-11α-BSA sera. The progesterone-3-[ 125I] double-antibody RIA, however, is more convenient and less expensive than the [ 3H]-progesterone RIA method.
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