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Abstract
A two-antibody immunoassay for glucagon has been described. The method is sensitive to as little as 0.1 ng of glucagon, permitting reliable measurements of glucagon in 1 ml of human serum. The use of a proteolytic enzyme inhibitor (Trasylol) made satisfactory recoveries of added glucagon possible. The average fasting serum immuno-reactive glucagon (IRG) concentration of 5 normal subjects was 0.44 ng/ml (0.40 to 0.46; 10 determinations). The oral administration of 100 g of glucose caused a small, but statistically significant increase in serum IRG, while the intravenous injection of glucose was followed by a prompt and statistically significant decline. The insulinogenic response to oral glucose was greater than the response to intravenous glucose. The significance of these results is discussed.
Published Version
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