Abstract
Biological dosimetry (biodosimetry) is based on the investigation of radiation-induced biological effects (biomarkers), mainly dicentric chromosomes, in order to correlate them with radiation dose. To interpret the dicentric score in terms of absorbed dose, a calibration curve is needed. Each curve should be constructed with respect to basic physical parameters, such as the type of ionizing radiation characterized by low or high linear energy transfer (LET) and dose rate. This study was designed to obtain dose calibration curves by scoring of dicentric chromosomes in peripheral blood lymphocytes irradiated in vitro with a 6 MV electron linear accelerator (Mevatron M, Siemens, USA). Two software programs, CABAS (Chromosomal Aberration Calculation Software) and Dose Estimate, were used to generate the curve. The two software programs are discussed; the results obtained were compared with each other and with other published low LET radiation curves. Both software programs resulted in identical linear and quadratic terms for the curve presented here, which was in good agreement with published curves for similar radiation quality and dose rates.
Highlights
Biological dosimetry is an important method for estimating the dose of ionizing radiation absorbed by a person, and is based on biological endpoints modified after chronic or acute exposure to this physical agent [1]
Scoring of dicentrics in peripheral blood lymphocytes cultured in vitro is interpreted in terms of the absorbed radiation dose with reference to a dose-response calibration curve generated by irradiating blood samples from healthy, unexposed donors with different absorbed doses of a defined quality of radiation
Statistical analyses Dose-response calibration curves were constructed with the CABAS Software version 2.0 and Dose Estimate software version 4.1
Summary
Biological dosimetry (biodosimetry) is an important method for estimating the dose of ionizing radiation absorbed by a person, and is based on biological endpoints modified after chronic or acute exposure to this physical agent [1]. A number of studies have demonstrated a close correspondence between the yield of radiation-induced dicentrics and the absorbed radiation dose for either in vivo or in vitro exposures [1,2]. Scoring of dicentrics in peripheral blood lymphocytes cultured in vitro is interpreted in terms of the absorbed radiation dose with reference to a dose-response calibration curve generated by irradiating blood samples from healthy, unexposed donors with different absorbed doses of a defined quality of radiation. The yield of radiationinduced dicentrics is dose dependent, increasing linearly for small absorbed doses, and quadratically for high absorbed doses of low LET radiation. This is known as the linear-quadratic model. For high LET radiations, the shape of the dose response is linear, with no quadratic term [4,5]
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