Abstract
Diurnal variation of N-acetylserotonin in the retina was studied in male rats housed under a photoperiod of 12 h light and 12 h darkness (lights on at 06.00 h) for two weeks. N-acetylserotonin was extracted from the retina by ethyl acetate at pH ≤ 3 and was determined by radioimmunoassay. There is a diurnal rhythm of N-acetylserotonin in the retina with high at night and low in the daytime. It is suggested that N-acetylserotonin in the retina may contribute partly to the levels of circulating N-acetylserotonin and may play a role in the regulation of photomechanical changes in the eye of rats.
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