Abstract
The fluorescent Ca 2+ indicator Fura 2 was used to monitor Ca 2+ release induced by the Ins(1,4,5)P 3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-( tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca 2+ conditions, tuBHQ, Ag II and caffeine elicited Ca 2+ rises, indicating Ca 2+ release from internal stores. Prior addition of Ag II had no noticeable effect on the extent of release of Ca 2+ induced by tuBHQ. Stimulation of the cells with tuBHQ before either Ag II or caffeine, similarly had no effect on Ca 2+ released by these two agonists. It was concluded, therefore, that there is a third intracellular Ca 2+ store in bovine adrenal chromaffin cells, distinct and non-overlapping, from those sensitive to caffeine or Ins(1,4,5)P 3-Mobilizing agonists.
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