Abstract
A novel, portable, and disposable paper-based microbial fuel cells (MFC) generating electricity from harmless strain of Escherichia coli (E. coli) has been developed. This MFC is on-demand electricity generation activated by a drop of tap water in a dry anode containing the biofilm of E. coli. A low-cost, simple-structure MFC case was created by a single layer of paper filter with high order of folding, which could support interchangeable dry bacteria-loaded anodes. The paper filter was innovatively engineered to make an integrated air-cathode proton exchange membrane (ACPEM) by coating carbon nanotube mixed with potassium ferricyanide on one surface and spraying hydrophobic solution on the other surface, respectively. The E. coli biofilm pre-inoculated in the anodes made of activated carbon sheet was simply dried in the air for the sake of long-term storage and usage. The dry anodes can be simply set in the MFC case with the readily prepared ACPEM, which can be used for on-demand power generation within seconds after adding water with maximum power density of 8.8μW/cm2.
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