A disposable and cost-effective electrochemical DNA sensor using nanocomposite modified screen-printed gold electrode

Abstract

This research involved the preparation of an electrochemical biosensor using a disposable screen-printed gold electrode (SPGE) for the DNA hybridization detection. An electrochemical DNA biosensor was successfully fabricated based on DNA probe tagged with methylene blue (MB) as redox hybridization indicator, was immobilized on the nanocomposite modified electrode. The modified SPGE was characterized by using cyclic voltammetry (CV) and scanning electron microscopy (SEM) with energy dispersion x-ray spectroscopy (EDS). The current signal of target DNA hybridization was monitored using differential pulse voltammetry (DPV). These DNA biosensor showed a good current response with the complementary target DNA concentration range from 1.0 x 10-11 to 1.0 x 10-7 M. This fabricated genosensor could also be regenerated easily and can be reused 36 times for hybridization studied.