Abstract
A specific radioimmunoassay has been developed for the direct measurement of 6β-hydroxycortisol in human urine. Preliminary purification of the steroid by either chromatography or extraction was not necessary. The assay had a sensitivity of 25 pg, an intra-assay variation of 7.3%, and an inter-assay variation of 8.6%. The specificity of the assay was checked using chromatography. The mean 24 hr urinary output of 6β-hydroxycortisol measured in normal males was 286 μg and in normal females 233 μg but the difference was not statistically significant. The effect of rifampicin and idomethacin on the urinary excretion of 6β-hydroxycortisol was investigated.
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