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Abstract
A method is described for the determination of tertiary amyl alcohol in plasma. The procedure employs direct Injection of heparinized plasma, separation by gas-liquid-solid chromatography, and subsequent quantification via selective ion monitoring mass spectrometry. Tertiary amyl alcohol is determined by monitoring fragment ions at m/e 59 and m/e 73. Recoveries averaged 99% _+ 9% (-+S.D.) with a quantitative detection limit (2.5 x noise) of 0.1 /Jg/ml. The coefficient of variation for five replicate determinations at various plasma concentrations of tertiary amyl alcohol varied from 3.8% at 160/~g/ml to 8.5% at 0.16/Jg/ml. Analyses can be accomplished with as little as 50 ~1 of blood In approximately 15 minutes.
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