Abstract
A new dinuclear-copper(II) complex (Cu-L2) with two ammonium arms based on bis-2-((pyridin-2-ylmethylamino)methyl)phenol as the coordinated unit was synthesized. The complex exhibited high affinity with pyrophosphate (PPi) in aqueous solution. Using pyrocatechol violet (PV) as a colorimetric indicator, the indicator displacement assay (IDA) was carried out to determine the binding constant between the complex and PPi. A ca. 784-fold enhancement was founded when compared Cu-L2 to ammonium-free counterpart (Cu-L1). The new dinuclear complex was successfully used to monitor the hydrolysis of PPi catalyzed by pyrophosphatase (PPase). Furthermore, by employing esculetine as a fluorescent indicator, the complex was also used to detect PPi released during polymerase chain reaction (PCR).
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