Abstract
The diethyl pyrocarbonate (DEP) 1 1 Abbreviations: DEP, diethyl pyrocarbonate; DNA, deoxyribonucleic acid; hrRNA, heavy ribosomal RNA; lrRNA, light ribosomal RNA; RNA, ribonucleic acid; rRNA, ribosomal RNA; SDS, sodium dodecyl sulfate; sRNA, soluble RNA; TMV, tobacco mosaic virus; Tris, tris/hydroxymethyl/aminomethane. method, a new procedure developed for ex tracting undegraded nucleic acids from plant tissues, can be used to isolate host nucleic acids from TMV-infected tobacco leaves without extracting the TMV-RNA.
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