Abstract
Objectives: The known model of blood coagulation involves a series of zymogen activation reaction sequences. At each stage, a zymogen is converted to an active protease by cleavage of one or more peptide bonds in the precursor molecule. The aim of this study was to investigate amino acid profiles during coagulation process in different conditions in vitro. Methods: Samples of serum and plasma (treated by EDTA or citrate) were obtained from healthy donors and from patients with Phenylketonuria (PKU). Amino acid profiles analyzed with reverse phase HPLC column. Results: There were no differences between two plasma amino acid levels which were obtained by EDTA and acid citrate (p>0.05). Serum aspartate (asp), glutamate (glu), serine (ser), histidine (his) and phenylalanine (phe) levels were significantly higher in serum than plasma (p<0.05). This significant difference was not observed in patients with PKU. Conclusion: As a result the enzymatic reactions of coagulation process generate some aminoacids and these reactions take place in an appropriate chemical microenvironment. This microenvironment can be used to clarify the stages of coagulation cascades with further studies.
Highlights
Blood coagulation is a complex physiological process which leads to the formation of fibrin clot through the proteolytic action of thrombin on fibrinogen
The activated partial thromboplastin time test and the prothrombin time (PT) tests were performed by manual methods depending on fibrin clot formation
Amino acid analysis were performed by a well-recognized high-performance liquid chromatography (HPLC) method on Agilent 1100 HPLC described by manufacturer with flourosence detection and reverse phase HPLC column
Summary
Blood coagulation is a complex physiological process which leads to the formation of fibrin clot through the proteolytic action of thrombin on fibrinogen. When blood is collected from the body and placed in a test tube, it clots quickly. The formation of thrombin generation during blood coagulation has become the most reliable approach to assess the global integrity of this complex process. All cellular and soluble plasma participants to this reaction have to be present to give the full picture of the physiology of this multifactorial reaction, in their natural whole blood environment[1], in vitro conditions may give some important clues to this reaction. O. Evliyaoğlu et al New insights into blood coagulation
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
