A Different Approach to Augment Pigment Production and its Extraction from Kocuria flava by Using Ultrasound Technique

Abstract

This research work reports the mapping of an ultrasonic bath for production of yellow bacterial pigment from Kocuria flava species. The main objective of this work was to intensify pigment production from the isolated bacteria and to reduce the time required for pigment production by using ultrasound. The isolated bacterium was grown at 37°C for 48 hrs in a rotary shaker at 180 RPM. The influence of different parameters like frequency, time, power, position, and the depth of ultrasound bath on the pigment productivity were investigated to get maximum yield. The initial pigment yield of 18.18 μg/mL increased to 86.33 μg/mL after ultrasound treatment at the optimized parameters of 24 kHz frequency, time duration of 20 min, power input at 120 W, and a height of 2.54 cm from bottom of the bath. Thus, a significant increase in the pigment production by ultrasonic treatment shows the advantages of ultrasound treatment on the production of natural pigment. Hence, this study can prove as a fundamental study for other ultrasound bath to achieve maximum productivity for any bacterial pigment