A diet supplemented with hazelnut skin changes the microbial community composition and the biohydrogenation pattern of linoleic acid in the rumen of growing lambs

Matteo Daghio,Carlo Viti,Federica Mannelli,Mariano Pauselli,Antonio Natalello,Giuseppe Luciano,Bernardo Valenti,Arianna Buccioni

doi:10.1080/1828051x.2021.1955020

Matteo Daghio, Carlo Viti + Show 6 more

Open Access

https://doi.org/10.1080/1828051x.2021.1955020

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Abstract

The reuse of by-products from the agro-industry in animal feeding is desirable to increase the sustainability of the productions. Hazelnut skin is a by-product of the food industry and due to the high content in crude fat and phenolic compounds can be potentially used in ruminants’ diet. In this study, we characterised and compared the microbial communities of rumen digesta (RD) from lambs fed a diet supplied with hazelnut skin vs. a control diet. Microbial DNA was extracted and high-throughput sequencing of 16S rRNA gene amplicons was performed. Six genera ([Eubacterium] nodatum group, Acidaminococcus, Dialister, Erysipelotrichaceae UCG-002, Megasphaera, and Olsenella) showed a positive correlation with the concentration of C18:1 t10, and their relative abundance was higher in the RD from lambs fed the control diet, compared to the RD from lambs fed the diet supplied with hazelnut skin. The results suggest that the relative abundance of microorganisms correlated to C18:1 t10 production in the rumen of animals fed a concentrate-based diet can be reduced by the administration of hazelnut skin. Highlights A diet with hazelnut skin affected the microbial community in the rumen of lambs The relative abundance of the genus Dialister decreased with hazelnut skin Administration of hazelnut skin can be a strategy to reduce the C18:1 t10/t11 ratio

Highlights

The use of alternative human-inedible feed resources is desirable for sustainable meat production
The diversity indexes calculated for the amplicon sequence variants (ASVs) abundance indicated a higher alpha-diversity in the rumen digesta (RD) of HS compared to control group (CON) (Figure 1)
An non-metric multidimensional scaling (NMDS) plot showed that the microbial community in the RD of HS was different compared to the community in CON (Figure 2)

Summary

Introduction

The use of alternative human-inedible feed resources is desirable for sustainable meat production. The use of agro-industrial by-products in animal feeding represents an excellent opportunity to shift towards a sustainable livestock system, with the added advantage to reduce the cost associated with their disposal (Correddu et al 2020). Hazelnut skin is a by-product rich in crude fat and phenolic compounds (Caccamo et al.2019). The main phenolic compounds in hazelnut skin are gallic acid, procyanidin dimers and trimers, flavan3-ols, flavonols, and hydrolysable tannins, such as glansreginin A, B, and C (Del Rio et al 2011). Phenolic compounds are plant secondary metabolites able to modulate the activity of rumen microbiota and to influence the biohydrogenation (BH) of unsaturated fatty acids in the rumen (Vasta et al 2019).

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