Abstract
Black root rot (BRR) is a major seedling disease of cotton in Australia. The disease is caused by Berkeleyomyces rouxiae (formerly known as Thielaviopsis basicola), which is morphologically identical to B. basicola. In this diagnostic guide, we share current work and knowledge regarding B. rouxiae in cotton in Australia. This guide includes comprehensive signs and symptoms to allow correct identification of the disease in the field. Methods and tricks to isolate and purify the pathogen from soil and diseased cotton are also discussed. Additionally, available molecular approaches for accurate identification of B. rouxiae are summarized. Last but not least, methods to preserve and assess pathogenicity of B. rouxiae on cotton are presented.
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