Abstract
Various chamber designs for infecting natural populations of Glossina pallidipes, G. longipennis, and G. fuscipes fuscipes with the entomopathogenic fungus Metarhizium anisopliae were tested in the field. All three species of tsetse flies entered the chambers and became infected with the fungus. Mortality attributed to infection by M. anisopliae ranged from 0 to 76% for G. pallidipes/G. longipennis and from 0 to 80% for G. fuscipes. One design proved to be more efficient than the others in permitting the passage of flies and contaminating them with fungal conidia. Dry conidia of M. anisopliae in the infection chamber retained their infectivity for more than 21 days in the field.
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